N-(7-Dimethylamino-4-methyl-coumarinyl) maleimides (DACM) : Novel Fluorescent Thiol Reagents
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The accessibility of the thiol groups on G- and F-actin of rabbit muscle.
The accessibility of the cysteine residues of actin from rabbit muscles to the thiol-targeted reagent 7-dimethylamino-4-methyl-(N-maleimidyl)coumarin (DACM) was investigated. Under conditions where the actin is in the unpolymerized form (G-actin), the most reactive thiol group was Cys-257, suggesting that it was located on the surface of the actin molecule. The selective modification of Cys-374...
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ژورنال
عنوان ژورنال: CHEMICAL & PHARMACEUTICAL BULLETIN
سال: 1975
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.23.1385